nCal (dose response curve) and prc (paired response curve)

These two R packages are hosted on on and In addition, here is a FAQ focused on installation and graphical user interface (GUI) of nCal.



  • Fong, Y, Yu, X. (2016) Transformation Model Choice in Nonlinear Regression Analysis of Serial Dilution Assays, Statistics in Biopharmaceutical Research, 8(1):1-11.
  • Cumberland, W.N., Fong, Y†, Yu, X., Defawe, O., Frahm, N., DeRosa, S. (2015) Nonlinear calibration model choice between the four and five parameter logistic models, Journal of Biopharmaceutical Statistics, 25(5):972-983.
  • Eckels, J., Nathe, C., Nelson, E., Shoemaker, S., Van Nostrand, E., Ashley, V., Yates, N., Harris, L., Bollenbeck, M., Fong, Y., Tomaras, G., Piehler,B. (2013) Analysis, quality control and secure sharing of Luminex immunoassay data using the open source LabKey Server system. BMC Bioinformatics, 14(1):145-162.
  • Fong, Y., Wakefield, J., DeRosa, S., Frahm, N. (2012) A robust Bayesian random effects model for nonlinear calibration problems, Biometrics, 68(4):1103--1112.
  • Fong, Y., Sebestyen, K., Yu, X, Gilbert, P. and Self, S. (2013) nCal: a R package for nonlinear calibration, Bioinformatics, 29(20):2653-2654.
  • Defawe OD, Fong Y, Pickett M, Vasilyeva E, Carter DK, Gabriel E, Frahm N, McElrath M.J., De Rosa SC. (2012) Optimization and qualification of a multiplex bead array to assess cytokine and chemokine production by vaccine-specific cells. Journal of Immunological Method, 382(1):117-128.