BubR1-GLEBs domain. BubR1 is a multiple functional protein that plays key roles implicated in mitotic checkpoint control, mitotic timing, and regulating kinetochore-microtubule attachment. We found that certain GBM isolates are sensitive to inhibition of BubR1's GLE2p-binding sequence (GLEBS) domain. We have shown, for example, that in BubR1 knockout cells that BubR1's GLEBs domain becomes essential for kinetochore-microtubule attachment and viability only after oncogenic activation of the RTK/Ras-pathway (Ding et al., Cancer Discovery, 2013). Importantly, we were able to find a key molecular indicator on the kinetochore that predicts whether GBM or other cell types will be sensitive to loss of BubR1's GLEBs domain function. We are currently working with Dr. Jim Olson's group (Clinical Research Division) to identify molecules that will interfere with BubR1's GLEBs domain activity.