In the Tsukiyama Lab, we are interested in finding out how chromatin is regulated. Our current interest centers around the functions of multi-subunit complexes containing ISWI. The complexes in this class are highly conserved from yeast to humans, and use the energy of ATP hydrolysis to move nucleosomes around in vitro. However, it is poorly understood what they do in vivo. We believe that we will be able to learn new principles in chromatin regulation by finding out how ISWI complexes function. We are taking advantage of many tools for molecular genetic and biochemistry available in our model system, S. cerevisiae.