SHOU LAB -- Publications


Widder S, Allen RJ, Pfeiffer T, Curtis TP, Wiuf C, Sloan WT, Cordero OX, Brown SP, Momeni B, Shou W, Kettle H, Flint HJ, Haas AF, Laroche B, Kreft JU, Rainey PB, Freilich S, Schuster S, Milferstedt K, van der Meer JR, Groszkopf T, Huisman J, Free A, Picioreanu C, Quince C, Klapper I, Labarthe S, Smets BF, Wang H; Isaac Newton Institute Fellows, Soyer OS.  2016. Challenges in microbial ecology: building predictive understanding of community function and dynamics. ISME J. doi: 10.1038/ismej.2016.45

Lindemann SR, Bernstein HC, Song HS, Fredrickson JK, Fields MW, Shou W, Johnson DR, Beliaev AS.  2016. Engineering microbial consortia for controllable outputs. ISME J. doi: 10.038/ismej.2016.26

Shou, W. 2015. Acknowledging selection at sub-organismal levels resolves controversy on pro-cooperation mechanisms. eLife 10106.

Waite AJ, Cannistra C, Shou W. 2015. Defectors Can Create Conditions That Rescue Cooperation. PLoS Comput Biol. 11(12)e:1004645.
News coverage - interview with first author Adam Waite
Fred Hutch Science Spotlight

Shou, W, Bergstrom C, Chakraborty A, Skinner, F. 2015. Theory, models and biology. eLife 4:e07158.
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Waite AJ, Shou W. 2014. Constructing synthetic microbial communities to explore the ecology and evolution of symbiosis. In Engineering and Analyzing Multicellular Systems: Methods and Protocols. Springer: New York; 27-38.
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Green R, Shou W. 2014. Modeling Community Population Dynamics with the Open-Source Language R. In Engineering and Analyzing Multicellular Systems: Methods and Protocols. Springer: New York; 209-231.
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Kang S, Kahan S, Momeni B. 2014. Simulating Microbial Community Patterning Using Biocellion. In Engineering and Analyzing Multicellular Systems: Methods and Protocols,. Springer: New York; 233-253.
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Momeni B, Waite AJ, Shou W. 2013. Spatial self-organization favors heterotypic cooperation over cheating. eLife, 2: e00960.
Full Text (6.9 MB) Hutch Science Spotlight

Momeni B, Brileya KA, Fields MW, Shou W. 2013. Strong inter-population cooperation leads to partner intermixing in microbial communities. eLife, e00230.
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(6.9 MB) Hutch Science Spotlight

Waite AJ, Shou W. 2012. Adaptation to a new environment allows cooperators to purge cheaters stochastically. Proceedings of the National Academy of Sciences, 109: 19079-19086.
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Momeni B, Shou W. 2012. Cryosectioning Yeast Communities for Examining Fluorescence Patterns.. | JoVE Video. Journal of Visualized Experiments, 70: e50101.

Momeni B, Chen C-C, Hillesland KL, Waite AJ, Shou W. 2011. (Review) Using artificial systems to explore the ecology and evolution of symbioses. Cellular and Molecular Life Sciences, 68: 1353-1368.
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Shou W, Ram S, Vilar JMG. 2007. Synthetic cooperation in engineered yeast populations. Proceedings of the National Academy of Sciences, 104: 1877-1882.
Full text (135 KB) Supplementary (830 KB)

Azzam R, Chen SL, Shou W, Mah AS, Alexandru G, Nasmyth K, Annan RS, Carr SA, Deshaies RJ. 2004. Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. Science, 305: 516-519.
Full text (1.3 MB)) Supplementary (775 KB)

Park CJ, Song S, Lee PR, Shou W, Deshaies RJ, Lee KS. 2003. Loss of CDC5 function in Saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics, 163: 21-33.
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Shou W, Azzam R, Chen SL, Huddleston MJ, Baskerville C, Charbonneau H, Annan RS, Carr SA, Deshaies RJ. 2002. Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Molecular Biology, 3: 3.
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Shou W, Verma R, Annan RS, Huddleston MJ, Chen SL, Carr SA, Deshaies RJ. 2002. Mapping phosphorylation sites in proteins by mass spectrometry. Methods in Enzymology, 351: 279-296.
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Chen SL, Huddleston MJ, Shou W, Deshaies RJ, Annan RS, Carr SA. 2002. Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Molecular & Cellular Proteomics, 1: 186-196.
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Shou W, Deshaies RJ. 2002. Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genetics, 3: 4.
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Traverso EE, Baskerville C, Liu Y, Shou W, James P, Deshaies RJ, Charbonneau H. 2001. Characterization of the Net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. The Journal of Biological Chemistry, 276: 21924-21931.
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Shou W, Sakamoto KM, Keener J, Morimoto KW, Traverso EE, Azzam R, Hoppe GJ, Feldman RM, DeModena J, Moazed D, Charbonneau H, Nomura M, Deshaies RJ. 2001. Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Molecular Cell, 8: 45-55.
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Lippincott J, Shannon KB, Shou W, Deshaies RJ, Li R. 2001. The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. Journal of Cell Science, 114: 1379-1386.
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Shou W, Seol JH, Shevchenko A, Baskerville C, Moazed D, Chen ZW, Jang J, Shevchenko A, Charbonneau H, Deshaies RJ. 1999. Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell, 97: 233-244.
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Straight AF, Shou W, Dowd GJ, Turck CW, Deshaies RJ, Johnson AD, Moazed D. 1999. Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell, 97: 245-256.
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Shou W, Dunphy WG. 1996. Cell cycle control by Xenopus p28Kix1, a developmentally regulated inhibitor of cyclin-dependent kinases. Molecular Biology of the Cell, 7: 457-469.
Full Text (2.96 MB)