Because you most likely have not completed your order.
YOU DO NOT PROVIDE ANY PAYMENT INFORMATION ON THIS SITE. YOU CANNOT GET YOUR CELLS UNTIL YOU HAVE PROCESSED AN ORDER.
Why? Because the terms and conditions serves as the MTA between the Hutch and you. Until that is done, no vials leave the Hutch.
Under these conditions we offer a replacement or refund:
Using your own FedEx account # will save you money! It is a minimum of 60$ if we have to use our own to send them out!
Very few of our medulloblastomas have been successful in culture, and they have a reputation for being very difficult to be grown in this way. Under no circumstances do we advise putting our orthotopic PDX cells into TC. If you are interested in growing these cell lines in TC please purchase those lines and note our return policy does not include the use of our orthotopic PDX cell lines in TC.
Click here for the protocol
Laminin coating was done in the protocol we follow.
But we are not just blindly following the protocol. Most of our lines die if they do not have laminin coated plates, so it is necessary. A couple of our lines can grow as clumps or spheres in suspension but that is not ideal.
Useful cell lines that grow in suspension tend to float around as single cells. Clumps or spheres of cells are much harder to work with. You have to disperse the cells before you do anything and that is not as easy as it sounds.
At this time we are not offering back-ordering of cell lines as tumor onset can be quite long for several of our lines. Please use the alert system to be notified by email when a sample becomes available (it is where the "place in cart" button would be if it were in stock).
All listed prices are for academia. Industry queries should be addressed to the office of Industry Relations and Technology Transfer:
Fred Hutchinson Cancer Research Center
Industry Relations and Technology Transfer Office
P.O. Box 19024, J2-110
Seattle, WA 98109-1024
823 Yale Ave. N.
Seattle, WA 98109
For general inquiries, contact:
Fax: (206) 667-4732
Mail stop: J2-110
Tumor onset can range anywhere from 3 weeks to 6 months depending on the tumor model. Tumor cells coming out of frozen sometimes take a little longer to form observable tumors in mice, but once a tumor is collected and passaged the time to onset should decrease slightly and become more predictable.
For more specific tumor onset time, please contact us with your exact cell line and passage information!
We currently use our NSG breeding colony originally started from NSG mice from Jax. We have used Nu/Nu mice as well but have moved completely to NSG because of their greater degree of immunosuppression and ease of maintaining a breeding colony.
The best way to expand the PDX mouse derived line is by orthotopic xenograft. One vial can easily supply enough material for implantation into 5-10 mice, and a single tumor grown in mice can supply many more than that. Expansion in this way will provide enough material for experiments in tissue culture or in flank xenografts, as well as passaging in mice to keep the model going.
A vial probably does not contain enough cells to create a flank xenograft. Similar to expanding in culture conditions our tumor models only grown in the microenvironment of the brain, and growing these as flank tumors may fundamentally change the model.
A vial may contain enough cells to begin a cell culture, but our models have never been exposed to culture conditions which might change the behavior of this model. Human tumors are also have a high failure rate in culture and our lab will not supply vials to people who put our mouse lines into TC. Under no circumstances do we advise putting our orthotopic PDX cells into TC. If you are interested in growing these cell lines in TC please purchase those lines and note that our return policy does not include the use of our orthotopic PDX mouse cell lines in TC.
Here are links to our current SOPs in PDF version:
All of our tumor models were prepared from a human patient tumor sample that never saw culture conditions and were grown exclusively as orthotopic xenograft in mice.