Welcome to the Hadland lab. Our lab’s focus is in understanding the origin of blood and immune cells during development. We are particularly interested in determining the mechanisms by which hematopoietic stem cells (HSC) arise and how they acquire their unique properties, such as the capacity for life-long self-renewal and multilineage hematopoietic reconstitution following transplantation. Using mouse models, pluripotent stem cells, and engineered platforms to recapitulate aspects of HSC development in vitro, our goal is to unravel the dynamic intercellular interactions and signal pathways governing HSC genesis. Our long-term objective is to apply knowledge of embryonic HSC development toward de novo HSC generation and expansion in vitro, for the purposes of disease modelling, drug discovery, gene editing, and cellular therapies in blood and immune disorders.
Clonal analysis of embryonic hematopoietic stem cell precursors using index sorting combined with endothelial cell niche co-culture. Hadland BK, Varnum-Finney B, Nourigat-McKay C, Flowers D, Bernstein I.
A common origin for B-1a and B-2 lymphocytes in the clonal pre-HSC. Hadland BK, Varnum-Finney B, Mandal PK, Rossi DJ, Poulos MG, Butler JM, Rafii S, Yoder MC, Yoshimoto M, Bernstein ID.