Single Cell Transcriptomics

Characterizing the transcriptome of individual cells is fundamental to understanding complex biological systems. We validated a fully-integrated and robust droplet-based system that enables 3’ mRNA digital profiling of thousands of single cells in a highly multiplex fashion. Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min, with ∼50% cell capture efficiency.

Screen Shot 2017-02-09 at 4.32.05 PM

To demonstrate the system’s technical performance, we collected transcriptome data from ∼250k single cells across 29 samples. We validated the sensitivity of the system and its ability to detect rare populations using cell lines and synthetic RNAs. We profiled 68k peripheral blood mononuclear cells to demonstrate the system’s ability to characterize large immune populations:

Screen Shot 2017-02-09 at 4.36.34 PM

Finally, we used sequence variation in the transcriptome data to determine host and donor chimerism at single-cell resolution from bone marrow mononuclear cells isolated from transplant patients.

Related News